ABSTRACT
<p><b>OBJECTIVE</b>To screen and determine the regions of copy number variation (CNV) associated with hepatocellular carcinoma (HCC) using SNP array and fluorescence quantitative PCR.</p><p><b>METHODS</b>The CNV from HCC cell line TJ3ZX-01 was analyzed using GeneChip Human Mapping 500K SNP array. According to the data obtained by SNP array analysis, four candidate amplification regions were verified in 41 primary HCC samples by fluorescence quantitative PCR.</p><p><b>RESULTS</b>Four regions of copy number amplification at 1q21.2, 1q22 approximately 23.1, 7p22.1 and 22q13.1 were detected by SNP array analysis. The four candidate amplicons occurred in 56.1% (23/41) of HCC samples at 1q21.2; 80.5% (33/41) at 1q22 approximately 23.1; 75.6% (31/41) at 7p22.1 and 31.7% (13/41) at 22q13.1 analyzed with sequence tagged site (STS) markers by quantitative PCR.</p><p><b>CONCLUSION</b>In four candidate amplification regions selected by SNP array analysis and detected by fluorescence quantitative PCR, three amplification regions show increased copy number in more than 50.0% HCC tissues. This result indicates that these amplification regions are associated with pathogenesis of hepatocellular carcinoma.</p>
Subject(s)
Female , Humans , Male , Middle Aged , Carcinoma, Hepatocellular , Genetics , Pathology , Cell Line, Tumor , Chromosomes, Human, Pair 1 , Genetics , Chromosomes, Human, Pair 22 , Genetics , Chromosomes, Human, Pair 7 , Genetics , DNA Copy Number Variations , Genetics , Liver Neoplasms , Genetics , Pathology , Oligonucleotide Array Sequence Analysis , Polymerase Chain Reaction , Methods , Polymorphism, Single Nucleotide , Sequence Tagged SitesABSTRACT
<p><b>OBJECTIVE</b>In order to clarify the genetic background of Pinellia ternata germplasm resources in China, the chromosomal constitution and cytogeographical distribution of P. ternata were investigated in 27 different populations among 16 provinces and regions in China systematically.</p><p><b>METHOD</b>Cytological and cytogeographical methods were used in the study.</p><p><b>RESULT</b>P. ternata in China is a polyploid complex, which contains septuploid (2n = 7x = 91) , octoploid (2n = 8x = 104) , nonuploid (2n = 9x = 117) and decaploid (2n = 10x = 130). Meanwhile the aneuploid series (2n = 92, 103, 105, 115) of a minority of P. ternata were also found.</p><p><b>CONCLUSION</b>The genetic differentiation and the phenomenon of ploidy miscellany commonly exist in the species of P. ternata in China, both for natural populations and cultivated populations. Toxicity and chemical components of different ploidy P. ternata should be clarified before the superior multiploid is selected for normalized plantation of the plant.</p>
Subject(s)
Aneuploidy , China , Chromosomes, Plant , Genetics , Ecosystem , Genetic Variation , Pinellia , Genetics , Plants, Medicinal , Genetics , PolyploidyABSTRACT
<p><b>OBJECTIVE</b>To explore the methylation status of 5' CpG island of fragile histidine triad (FHIT) gene in plasma and the expression of FHIT protein in cancer tissue of cervical cancer patients.</p><p><b>METHODS</b>Methylation-specific PCR (MS-PCR) was employed to examine methylation of FHIT gene in 151 plasma samples before treatment. The immunohistochemistry was used to the expression of FHIT protein in cancer tissues.</p><p><b>RESULTS</b>CpG island methylation of FHIT was detected in 31.13% of plasma samples. The expression of FHIT protein was decreased or discarded in 59.60% of cervical cancer tissues. Among them 47.78% was included in methylation positive samples.</p><p><b>CONCLUSION</b>CpG island methylation of FHIT gene in plasma plays an important role on cervical cancer, which results in decreased expression of FHIT protein. It can be used to diagnose and evaluate the effect of treatment to cervical cancers.</p>